In conventional IVF or ICSI, the ovaries of women are stimulated to produce multi follicular development which involves giving multiple injections over a period of 15 days. These injections are expensive and also cause some complications like hyper stimulation if not monitored properly. Also, there are women with conditions like polycystic ovaries who are prone to develop hyper stimulation. In such situations, it would be best if we could aspirate immature follicles, grow them in the laboratory to the mature stage and subsequently perform ICSI to fertilize them. This technique of maturing the oocytes from an immature stage in the laboratory is called in vitro maturation. The technique is still not perfect and the results obtained are low compared to IVF and ICSI.